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Use the imagination, creativity, and engineering problem solving to tackle biomedical science problems and learn something everyday.
In search of genuine inspiration.

Education

(2006 - 2010) B.S., Clinical Laboratory Science and Medical Biotechnology, School of Medicine, National Taiwan University, Taipei, Taiwan

(2010 - 2012) M.S., Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang-Ming University, Taipei, Taiwan

(2014 - 2019) Ph.D. Candidate, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

Research experience

2007 - 2010, thematic research in Teng's Group, Clinical Laboratory Science and Medical Biotechnology, National Taiwan University, Taiwan
Advisor: Prof. Teng, Lee-Jene

2010 - 2012, graduate study & research assistant in BioMicrofluidic Applications Laboratory (BioMAps), Research Center for Applied Sciences, Academia Sinica, Taiwan
Advisor: Research Fellow, Dr. Cheng, Ji-Yen

2012 - 2014, thematic research under Dr. Cheng, Ji-Yen, Academia Sinica
Research Topic: Microfluidic cell culture platform
Research project: Whole cell sensing platform

2014 - 2019, Graduate Research Assistant, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
Topic: Automated microfluidic platform for cell biology and bioengineering research.

2017 - 2020, DC1 Research Fellow for JSPS, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
Topic: Study of glioma infiltration in complex microenvironment on an automated microfluidic chip.

Work experience

2010 - 2010, Internship, National Taiwan University Hospital Clinical Laboratory, Taiwan

2013 - 2014, Alternative Civilian Service in Ministry of Health and Welfare, Taiwan

2014 - 2014, Research assistant to Dr. Cheng, Ji-Yen, Academia Sinica

Research interests

  • Microelectromechanical systems(MEMS)/Microfluidics in biomedical applications
  • Tissue Engineering
  • Multimodal Super-resolution Microscopy
  • Biophysics (Bioelectromagnetics & Membrane Biophysics)
  • Aptamer Chemistry and Applications
  • Lab-On-Chip/Point-of-Care Testing Technologies/In Vitro Diagnostics/Biosensors
  • Disaster Management/Rapid Response Medicare
  • Infectious Disease Control and Prevention
  • Biomineralization
  • Laboratory Automation/Semantics for microfluidics

Fellowship and Grant

2008 - 2009 Undergraduate Student Thematic Grant from National Science Council of Taiwan, Grant No.NSC97-2815-C-002-072-B

Grant topic: The Effect of Farnesol on the Lipase and Protease Activity of Staphylococcus aureus and Staphylococcus epidermidis. (NTD$48000)

2017 - 2020 Grands-in-Aid for Scientific Research (KAKENHI) to Research Fellowship for Young Scientists (DC1) from Japan Society for the Promotion of Science (JSPS), Japan

Grant topic: Study of glioma infiltration in complex microenvironment on an automated microfluidic chip. (17J00362(¥3.1M) 

Award

2008 National Taiwan University Department of Clinical Laboratory Science and Medical Biotechnology Theodore Memorial Scholarship
2009 Research Creativity Award from the National Science Council of Taiwan.
2012 National Yang-Ming University Annual Thesis Competition Excellent Award

Conference attendance

Oral Presentation, International Photonics Conference 2011 (IPC2011) (Dec.8-10, 2011, NCKU, Tainan, Taiwan)
Poster, The 20th Symposium on Recent Advances in Cellular and Molecular Biology (Feb.7-9, 2012, Kaohsiung, Taiwan)
Poster, The 17th Biophysics Conference, (May 23-25, 2012, Taipei, Taiwan)
Poster, The 4th International Symposium on Microchemistry and Microsystems (ISMM 2012), (Jun.10-13, 2012, Zhubei city, Hsinchu, Taiwan)
Poster, The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012), (Oct.28-Nov.1, 2012, Okinawa, Japan)
Poster, Advances in Microfluidics and Nanofluidics 2014 (AMN2014), (May, 2014, Academia Sinica, Taipei, Taiwan)
Technical presentation, NanoEngineering for Medicine and Biology 2016 (NEMB2016) (Feb, 2016, Houston, Texas, USA)
Poster presentation, Lab on chip world congress 2017. (Oct, 2017, California, USA)

Certificate

Medical Technologist, Taiwan (2011)
Emergency Medical Technician-1, Taiwan (2014)

Publication

  1. Hsieh-Fu Tsai, Kazumi Toda-Peters, Amy Q. Shen*, Glioblastoma adhesion in a quick-fit hybrid microdevice, Biomedical Microdevices, submitted
  2. Hsieh-Fu Tsai*, Joanna Gajda, Tyler F.W. Sloan, Andrei Rares, Amy Q. Shen*, Usiigaci: Label-free instance-aware cell tracking in phase contrast microscopy enabled by machine learning, SoftwareX, in revision
    • [Original Software Article]
    • [DOI:]
    • Github repo
    • Youtube introduction video
    • [Summary] Usiigaci is an all-in-one Python-based cell segmentation, tracking, data analysis pipeline to analyze cell migration under label-free phase contrast microscopy using machine learning methods.
  3. Hsieh-Fu Tsai, Alen Trubelja, Amy Q. Shen* and Gang Bao*, Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment, Journal of Royal Society Interface, 20170137 (2017)
    • [Review]
    • [DOI:10.1098/rsif.2017.0137]
    • Review some recent microfluidic platforms targeting research in tumor microenvironments and applications.
  4. Toshiaki Mochizuki, Yi-Jyun Luo, Hsieh-Fu Tsai, Akane Hagiwara and Ichiro Masai*, Cell division and cadherin-mediated adhesion regulate lens epithelial cell movement in zebrafish, Development144, 708-719 (2017)
    • [DOI:10.1242/dev.138909]
    • [Summary] Investigation of cadherin and mechanical force in regulating lens epithelial cell movement during early development of zebrafish lens. I was involved specifically in the experiment of UV laser ablation and observe the immediate force induced cell migration after a wound.
  5. Joshua J. Cardiel, Daisuke Takagi, Hsieh-Fu Tsai and Amy Q. Shen*, Formation and flow behavior of micellar membranes in a T-shaped microchannel, Soft Matter12, 8226-8234 (2016)
  6. Hsieh-Fu TsaiJi-Yen Cheng, Hui-Fang Chang, Tadashi Yamamoto, Amy Q. Shen*, Uniform electric field generation in circular multi-well culture plates using polymeric inserts, Scientific Reports6:26222 (2016)
    • [DOI:10.1038/srep26222]
    • [Summary] It is very difficult to create uniform electric fields from two electric potentials in a circular chamber due to differences of electric resistances. We employed a rational 3D computer aided design (CAD) approach of a microfluidic structure to equalize the electrical resistances in the chamber. A polymer insert with CAD structures can create uniform electric field in wells of multi-well plates or tissue culture dishes very much like a transwell insert. We hope this design of insert can be useful to push electric field stimultion into standard biomedical laboratory. Also the design strategy could be useful for different disciplines.
  7. Hsieh-Fu Tsai, Yi-Ching Tsai, Sharon Yagur-Kroll, Noa Palevsky, Shimshon Belkin, Ji-Yen Cheng*Water pollutant monitoring by a whole cell array through lens-free detection on CCD, Lab on a Chip, 151472-1480 (2015)
    • [DOI:10.1039/C4LC01189A]
    • [Summary] Created a miniaturized platform for bioluminescent whole cell sensor using commercial linear charge-coupled devices.
  8. Hsien-San Hou, Hsieh-Fu Tsai, Hsien-Tai Chiu, and Ji-Yen Cheng*, Simultaneous chemical and electrical stimulation on lung cancer cells using a multichannel-dual-electric-field chip, Biomicrofluidics8, 052007 (2014)
  9. Kai-Yin Lo, Yun Zhu, Hsieh-Fu Tsai, and Yung-Shin Sun, Effects of shear stresses and antioxidant concentrations on the production of reactive oxygen species in lung cancer cells, Biomicrofluidics, 7, 064108 (2013)
  10. Hsieh-Fu Tsai, Ching-Wen Huang, Hui-Fang Chang, Jeremy J.W. Chen, Chau-Hwang Lee, Ji-Yen Cheng*, Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under dcEF stimulation, PLoS ONE, 8(8), e73418 (2013)
    • [DOI:10.1371/journal.pone.0073418]
    • [Summary] found that CL1-5 lung adenocarcinoma cell electrotaxis is independent of EGFR signalling despite CL1-5 cells express an abundant amount of EGFR. Suggesting the fundamental hypothesis may required modification. 
  11. Hsieh-Fu Tsai, Shih-Wei Peng, Chun-Ying Wu, Hui-Fang Chang, Ji-Yen Cheng*, Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field, Biomicrofluidics6, 034116 (2012)
    • [DOI:10.1063/1.4749826]
    • Supplementary Information difficult to get from AIP, if supplementary information is needed please contact.
    • [Summary] multiple electric field with uniform flow field creation based on R-2R ladder network concept. Also found a easy method to achieve bubble free cell culture in thermoplastic microfluidic chips.
  12. Ji-Yen Cheng*, Mansoureh Z. Mousavi, Chun-Ying Wu, Hsieh-Fu Tsai, Blue light emission from a glass/liquid Interface for real-time monitoring of a laser-induced etching process, Journal of Micromechanics and Microengineering21, 075019 (2011)

Patent

  1. US Patent Appl. 15/759,734 (Pending) 3D POLYMERIC INSERT TO APPLY UNIFORM ELECTRIC FIELD IN CIRCULAR CULTUREWARE

就學經歷

(2003 - 2006)        高中 - 台灣 高雄市立高雄高中
(2006 - 2010)        學士 - 台灣 國立台灣大學 醫學院 醫學檢驗暨生物技術學系
(2010 - 2012)        碩士 - 台灣 國立陽明大學 生物醫學暨工程學院 生醫光電研究所
(2014 - now)         博士候選人 - 琉球(日本) 沖縄科学技術大学院大学 科学技術研究科

研究經驗

(2007 - 2010) - 大學專題研究 - 國立台灣大學醫學檢驗暨生物技術學系鄧麗珍教授實驗室

(2010 - 2012) - 碩士研究 - 中央研究院 應用科學研究中心 鄭郅言副研究員實驗室

(2012 - 2014) - 中央研究院 應用科學研究中心 鄭郅言研究員實驗室
          專題研究:微流道細胞實驗平台
          助理研究:先進生物感測器平台

(2014 - now) - 沖縄科学技術大学院大学 Micro/Bio/Nanofluidics Unit
          指導老師:Prof. Amy Shen
博士班研究:微流道細胞實驗平台、跨領域神經科學

(2017 - 2020) - 特別研究員 日本學術振興會 (DC1)

工作經驗

(2013 - 2014) - 醫療替代役 - 衛生福利部醫事司
(2014 - 2014) - 研究助理 - 中央研究院 應用科學研究中心 鄭郅言研究員實驗室

研究興趣

  • 微流體技術在生醫上的應用
  • 細胞趨電性機制
  • 奈米解晰度顯微技術
  • 生物物理(生物電磁、細胞膜物理)
  • 適體化學與應用
  • 鄰病檢測醫材
  • 災害反應
  • 傳染疾病控制與預防
  • 生物礦化

補助計畫

2008 - 2009 國家科學委員會 大專學生參與研究專題計畫 NSC97-2815-C-002-072-B

計畫名稱:Farnesol對金黃葡萄球菌與表皮葡萄球菌之脂解酶和蛋白酶活性之影響

2017 - 2020 日本学術振興会 特別研究員 (DC1) + 科学研究費補助金 特別研究員奨

研究課題:自動マイクロ流体チップ上での複雑微環境における神経膠腫細胞の浸潤機制研究  

得獎經歷

2008 國立台灣大學醫學檢驗暨生物技術學系蔡德頭紀念獎學金
2009 國家科學委員會大專學生研究創作獎
2012 國立陽明大學學術論文比賽醫學技術及工程類碩B組佳作

研討會

口頭報告, 2011 國際光電科技研討會 (IPC2011, Dec.8-10 NCKU, Tainan, Taiwan)
海報, 2012 第20屆細胞及分子生物新知研討會 (Feb.7-9, Kaohsiung, Taiwan)
海報, 2012 第17屆生物物理研討會 (May.23-25, Taipei, Taiwan)
海報, 2012 第4屆國際微化學及微系統研討會 (ISMM2012, Jun.10-13, HsinChu, Taiwan)
海報, 2012 第16屆國際化學及生物微系統研討會 (MicroTAS 2012, Oct 28-Nov.1, Okinawa, Japan)
海報, 2014 微流體和奈米流體新知研討會 (AMN2014, May. 21-23, Taipei, Taiwan)

執照

醫事檢驗師(2011)
初級緊急救護技術員(2014)
Ċ
Paul, Hsieh-Fu Tsai,
Sep 9, 2018, 4:32 AM