Cell toxicity of microfluidic materials

The tygon tubings that used by many microfluidic groups together with stainless tube interface has been discussed by many experts regarding to cell toxicity.

Tygon of PVC and peroxide or platinum cured silicone have FDA approval. But several groups reported some cells maybe sensitive to the tubings.

Working with cells, we have been using more chemically inert PTFE or ETFE tubings. 



PDMS toxicity

Personal communication with Prof. Takayama on treating PDMS
curing at 150degree for 1hr.
separate PDMS chip for sensitive cells a dedicated oven free from dirty silanes and organic solvent etc.
can also add treatment of PDMS by boiling in water

1. Extraction of PDMS 

Conditioning of PDMS for

Existing reviews





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