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Medium Preparation

Dulbecco's modified Eagle's medium (DMEM) for 1L(final medium)  for medium with 10% FBS
all the powder is dissolved in 700mL Type I water

1.5g/L sodium bicarbonate powder (1.5 to 2.2g/L)for 5% CO2 incubator
3.7g/L for 10% CO2 incubator
dissolve in 200mL Type I water
equilibrate by CO2 bubbling

Mix the two. (900mL)
if medium is too basic -> equilibrate with CO2 gas
verify pH by small aliquot

filter and storage.

 

Reference:
1. Bioresource Collection and Research Center, Taiwan
2. Culture of Animal Cells, Fresney
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