mountant

Vector laboratories

T-pro
太鼎

abcam
卓昇



Mounting media

  • An inexpensive and simple mounting media= 90% glycerol / 100 mM Tris pH 8.0. Combine 1 ml of 1 M Tris pH 8.0 and 9 ml glycerol, and mix well. This medium will not protect against photobleaching, but performs well for most experiments.
  • DABCO / Tris / Glycerol (DTG)=
    • 2.5% w/v DABCO, e.g. Sigma #D-2522
      50 mM Tris pH 8.0
      90% glycerol
    • For 50 ml, use 1.25 g DABCO, 45 ml glycerol, 2.5 ml 1 M Tris pH 8.0, and 2.5 ml ddH2O.
    • Combine components, dissolve DABCO by warming to 70° C., mix, aliquot and store at -20° C.
    • Protects well against photobleaching.
  • 'Prolong' from Molecular Probes is extremely effective against photobleaching and it hardens for semi-permanent storage at -20° C

cold spring harbor protocols


Comments