Coverglass
Marienfeld 24x60mm high precision No.1.5H coverslip chemical resistant borosilicate glass D263 M thickness 0.170mm+/- 0.005mm slides or coverslips used in fluorescence microscopy are required to be extremely clean. newly opened coverslips may look clean but may have a thin film of grease that may interfere with processing steps and tissue culturng. so the coverslips need to be cleansed with acid or base solutions. Coating procedures such as gelatin, aminoalkylsilane or poly-L-lysine solution to promote adhesion of cells to glass surface. gelatin or aminoalkylsilane is usually used for tissue sections or small organisms, whereas poly-L-lysine is routinely used for culturing cells. CSHL protocols some other links Polystyrene slides: tissue culture treated polystyrene slides can be used to grow adherent cells. Thermanox Thermanox are made from members in polyolefin family. It is highly resistant to most chemicals, alcohols, aldehydes, hydrocarbons, dilute acids (<10%) and dilute alkalis (<2%). limited resistance to chlorinated hydro-carbons. Not resistant to concentrated acids or bases. culture treated on one side for enhanced cell attachment and growth. auto-fluorescent in the range 380 to 545nm. autoclavable 20mines steam, 20minutes dry at 120℃ Rinzl optical clear vinyl with refractive index of glass. ideal for wet mount and urines. not autofluorescent. Permanox slides permanox is a strong, biologically inert material resistant to non-chlorinated hydrocarbons, acetone and other EM reagents. permanox slides allow cell fixation and cell staining on the slide. non-autofluorescent. Coverslip for use in confocal microscopy. The thickness of the coverslip used in fluorescence microscopy is crucial. Most objectives are designed to be compatible with No.1.5 glass coverslips. a resources in nikon microscopy resource shows how crucial matching the thickness is imperative especially when using high numerical aperature objectives. some special objectives are designed with a correction collar to correct this. |